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The Efficacy of Multipex PCR in Comparioson with Agglutination and ELISA in Human Brucellosis Diagnostic تاریخ ثبت : 1392/03/08
طبقه بندي :
Titel : The Efficacy of Multipex PCR in Comparioson with Agglutination and ELISA in Human Brucellosis Diagnostic
Author : R. A. Lari, F. Fallah, A. Karimi, L. Gachkar, G. Angoti, A. Sanaei and L. Azimi
Journal : Iranian Journal of Clinical Infectious Diseases
Year : 2011
Volume : 6
Issue : 3
Pages : 143-146
Abstract :

Background: Brucellosis is a zoonotic disease and diagnosis of brucellosis is based on clinical symptoms and positive laboratory results. Since specificity of the serology tests are not not totally specific and sensitive, polymerase chain reaction (PCR) can be an alternative method in making the final decision in some doubtful cases.

Objective: In this study three diagnostic methods were compared in suspected patients with brucellosis in the endemic area, Mianeh, Iran. Methods: In this descriptive study, results of standard agglutination test (SAT) and specific immunoglobulin (Ig) G and IgM by enzyme-linked immunosorbent assay were compared with Multiplex PCR results in 100 patients with suspected brucellosis referred to the Imam Khomeini hospital, Mianeh, Iran. Their sera were collected and tested by SAT, ELISA and Multiplex PCR. DNA was extracted from serum samples and examined by Multiplex PCR involving specific primers for B. melitensis and B. abortus based on IS 711 in the brucella chromosome.

Results: we resulted 28 cases were positive for B. melitensis by Multiplex PCR technique and we found the results of SAT method and PCR were significant (P<0.05). Six samples were positive for B. abortus by PCR.

Conclusions: The results of present study showed that Multiplex PCR assay is a rapid and sensitive technique for diagnosis of brucellosis compared to SAT method. However it is more valuable when coupled with conventional methods.

Keywords : B. abortus, B. melitensis, B.suis, Enzyme linked immunosorbent assay
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